Percepción de usuarios de servicios de salud sobre la visibilidad social de los profesionales de enfermería durante la pandemia de COVID-19

Objetivo: Conocer la percepción de usuarios de los servicios de salud de tres comunas de Chile sobre la visibilidad social de los profesionales de enfermería durante el período de la pandemia de COVID-19. Material y Método: Estudio cualitativo descriptivo que utilizó la técnica del grupo focal. Se realizó un grupo focal con un muestreo de tipo intencionado conformado por siete participantes cuyos criterios de inclusión fueron ser usuarios del sistema de salud de Chile y mayor de 18 años. El reclutamiento de los participantes fue realizado por un afiche en redes sociales de la institución académica. La estrategia de análisis fue el de contenido. Resultados: Se encontraron tres temas y siete subtemas: la visibilidad de la profesión (imaginarios sociales, valoración de la labor, invisibilidad), primera línea (revaloración de los profesionales, seguridad y confianza) y género (profesión feminizada, invisibilidad). Conclusiones: Bajo el actual contexto de salubridad, la visibilidad de la enfermería se ha sostenido por situaciones que justamente son asociadas a la invisibilidad, salud mental, cargas laborales, cuestiones de género, entre otras. Se sugiere la realización de investigaciones que demuestren y difundan el importante rol de los profesionales de enfermería en la sociedad y promuevan su reconocimiento.

Objetivo: Conhecer a percepção de usuários dos serviços de saúde de três municípios do Chile sobre a visibilidade social dos profissionais de enfermagem durante o período da Pandemia da COVID-19. Material e Método: Estudo qualitativo descritivo que utilizou a técnica de grupo focal. Foi realizado um grupo focal com uma amostragem do tipo intencional, composta por sete participantes, cujos critérios de inclusão foram ser usuários do Sistema de Saúde do Chile e maiores de 18 anos. O recrutamento dos participantes foi realizado por um poster nas redes sociais da instituição acadêmica. A estratégia de análise foi de conteúdo. Resultados: Foram encontrados três temas e sete subtemas: a visibilidade da profissão (imaginários sociais, valorização do trabalho, invisibilidade), primeira linha (reavaliação dos profissionais, segurança e confiança) e gênero (profissão feminizada, invisibilidade). Conclusões: No contexto atual da saúde, a visibilidade da enfermagem tem sido sustentada por situações que se associam justamente à invisibilidade, saúde mental, carga de trabalho, questões de gênero, entre outras. Sugere-se a realização de pesquisas que demonstrem e divulguem o importante papel dos profissionais de enfermagem na sociedade e promovam seu reconhecimento.

Objective: To know the perception of users of health services in three communes of Chile regarding the social visibility of nursing professionals during the period of the COVID-19 Pandemic. Material and Method: Qualitative study with a descriptive design that used the focus group technique. A focus group was carried out with an intentional sampling, made up of seven participants whose inclusion criteria were: being users of the Chilean Health System and, older than 18 years. The recruitment of the participants was conducted by a poster in social networks of the academic institution. The analysis strategy was content. Results: Three themes and seven sub-themes were found. The themes were, the visibility of the profession (social imaginaries, value of work, invisibility), first line (revaluation of professionals, safety, and trust) and gender (feminized profession, invisibility). Conclusions: Under the current context of health, the visibility of nursing has been sustained by situations that are precisely associated with invisibility, mental health, workload, gender issues, among others. It is suggested that research be conducted to demonstrate and disseminate the significant role of nursing professionals in society and promote their recognition.

Overall survival prediction in metastatic castration-resistant prostate cancer treated with radium-223

ABSTRACT Objective Radium-223(223Ra) is indicated for patients (p) with metastatic castration resistant prostate cancer (mCRCP). Objectives The aim of this study was to evaluate the role of baseline clinical variables associated with overall survival (OS) and toxicity of 223Ra. Its purpose was to identify the factors that can predict a better response to treatment and provide information regarding the most appropriate time for the application of 223Ra. Materials and Methods Prospective study in 40p with mCRPC treated with 223Ra. End points were OS, progression-free survival and time to progression. The follow-up parameters were: doses received, hemoglobin (Hb), absolute neutrophil count (ANC), platelet count (PC), prostate specific antigen (PSA), alkaline phosphatase (ALP), Visual Analogue Scale for pain, Eastern Cooperative Oncology Group (ECOG) and WHO's Cancer Pain Ladder. The use of other treatments was also evaluated. Results Median OS was 17.1 months(mo) (CI95%6.5-27.7); 26/40p received complete treatment of 223Ra, without reaching a median OS and 14p received incomplete treatment with a median OS 13.6mo(CI95%1.6-25.6). Median follow-up was 11.2mo (range:1.3-45.2). The univariate analysis showed that factors as VAS, ECOG, Hb and ALP values were independently associated with OS. First line treatment with 223Ra was started in 11/40p, while 19p had been heavily pre-treated and 13p received concomitant treatment. Conclusions 223Ra therapy require an adequate selection of patients to obtain the greatest clinical benefit. Low basal Hb, hight basal ALP, bone marrow involvement and an altered ECOG were the main factors that decreased OS in our patients. 223Ra should be considered relatively early in the course of treatment.

Utilidad de la 18F-FDG PET/TC en la valoración de tumores de la vaina nerviosa / The Value of 18F-FDG PET/TC in the Assessment of Peripheral Nerve Sheath Tumors

La 18F-FDG PET/TC tiene un papel importante en la evaluación de los tumores de la vaina nerviosa periférica, especialmente para determinar la posibilidad de malignidad y el sitio idóneo para la toma de biopsia. Se expone el caso de una mujer de 34 años de edad con diagnóstico de tumor de vaina nerviosa periférica, localizado en el mediastino posterior, que generó síndrome de vena cava superior y síndrome de Horner. Se realizó 18F-FDG PET/TC para hacer el diagnóstico diferencial entre benignidad y malignidad. Se encontró masa heterogénea con áreas hipermetabólicas que alcanzaban un SUVmax (valor de captación estándar máximo) de 8,5, hallazgos que sugerían origen maligno con diferentes grados de diferenciación. La biopsia de los lugares con mayor metabolismo arrojó el resultado de tumor maligno de vaina nerviosa periférica.

18F-FDG PET/CT is a useful imaging modality in the diagnosis and follow-up of peripheral nerve sheath tumors, especially in the assessment of tumor grade and biopsy guidance. The case of a 34-years-old woman diagnosed with peripheral nerve sheath tumor located in the posterior mediastinum that generated superior vena cava syndrome and Horner syndrome is presented. 18F-FDG PET/TC was performed to assess the possibility of malignancy. An 18F-FDG PET/CT was performed to determine whether it was benign or malignant, a heterogeneous mass with hypermetabolic areas with a maximum standardized uptake value (SUVmax) of 8.5 was found, and suggested malignancy with multiple grades of differentiation. A tumor biopsy from the region of higher metabolism was recommended with pathology result of malignant peripheral nerve sheath tumor.

El cuerpo carotídeo en la homeostasis de la glucosa / The carotid body in glucose homeostasis

Los cambios en los niveles de glucosa en la sangre periférica o central se detectan por sensores a la glucosa situados en: los núcleos hipotalámicos, el páncreas, el núcleo del tracto solitario, el sistema portal hepático y los receptores del cuerpo carotídeo. El cuerpo carotídeo o glomus caroticum es un órgano quimiosensorial localizado de forma bilateral en la vecindad de la bifurcación de la carótida común. Los cuerpos carotídeos son sensores polimodales, que detectan los niveles de oxígeno, pH, bióxido de carbono, temperatura, osmolaridad, potasio, glucosae insulina en la sangre que los irriga. El objetivo de esta revisión es describir los trabajos realizados por Ramón Álvarez-Buylla y colaboradores en el Laboratorio de Neuroendocrinología del Centro Universitario de Investigaciones Biomédicas de la Universidad de Colima, y de otros investigadores, relacionados con el estudio de los cuerpos carotídeos en la homeostasis de la glucosa. Los CCs responden a la hipoglucemia a través de la depleción de trifosfato de adenosina, o a cambios en el pH y pO 2, sin depleción de trifosfato de adenosina. Es cada vez más evidente que los CCs juegan un papel fisiológico importante en la homeostasis de este carbohidrato, y que el estrés hipóxico ejerce una influencia tónica en la secreción de diversos neurotrasmisores y hormonas como la arginina-vasopresina, el óxido nítrico, el factor neurotrófico derivado del cerebro, ácido gamma-aminobutírico, glucagón, cortisol y otros, que participan en el procesamiento central de los mecanismos contrarregulatorios en la homeostasis glucémica. Los CCs inician respuestas contrarreguladoras a la hipoglucemia con la participación de la hipófisis, las adrenales, el páncreas y el sistema simpático.

Changes in glucose levels in the peripheral or central blood are detected by sensors located on glucose: hypothalamic nuclei, the pancreas, the nucleus tractus solitarius, the hepatic portal system and carotid body receptors. The carotid body or glomus caroticum is a chemosensory organ located bilaterally in the vicinity of the bifurcation of the common carotid. The carotid bodies are polymodal sensors, which detect levels of oxygen, pH, carbon dioxide, temperature, osmolarity, potassium, glucose and insulin in the blood that irrigates. The objective of this review is to describe the work done by Ramon Alvarez-Buylla and colleagues in the Laboratory of Neuroendocrinology at the University Center for Biomedical Research at the University of Colima, and other researchers, related to the study of the carotid bodies in homeostasis glucose. CCs respond to hypoglycaemia through depletion adenosine triphosphate, or to changes in pH and PO2 without depletion adenosine triphosphate. It is increasingly evident that CCs play an important physiological role in the homeostasis of this carbohydrate, and hypoxic stress exerts a tonic influence on the secretion of various neurotransmitters and hormones such as arginine vasopressin, nitric oxide, neurotrophic factor derived from brain gamma-aminobutyric acid, glucagon, cortisol and others involved in the central processing contrarregulatorios mechanisms in glucose homeostasis. CCs initiate counter-regulatory responses to hypoglycemia involving the pituitary, adrenal, pancreas and the sympathetic system.

La somatostatina en el núcleo del tracto solitario comisural modula la retención de glucosa cerebral postestimulación anóxica de los quimioreceptores carotídeos en ratas / Somatostatin into the Commissural Nucleus Tractus Solitarius Modulates Brain Glucose Retention Post- Anoxic Stimulation of the Carotid Chemoreceptor in Rats

El núcleo del tracto solitario comisural (NTSc) es el centro de relevo de las fibras aferentes procedentes de los baro y quimiorreceptores carotídeos, por lo que modula la presión arterial y la glucemia ante los estímulos en dichos receptores. La estimulación anóxica con cianuro de sodio (NaCN) en los cuerpos carotídeos produce una respuesta hiperglucemiante. La somatostatina (SS) inhibe la secreción de la hormona del crecimiento y del glucagón lo que produce un efecto hipoglucemiante. La SS y sus receptores en el NTS tienen un efecto inhibidor. Se postula que la somatostatina modula la respuesta hiperglucemiante después de la estimulación de los quimiorreceptores carotídeos (QRC) con NaCN. En este trabajo, la infunsión de SS en el NTSc 4 min antes del estímulo anóxico de los QRC, disminuyó el reflejo hiperglucemiante y la retención de glucosa cerebral a los 10 min del estímulo anóxico. Se concluye que la SS en el NTSc modula la respuesta hiperglucemiante y la retención de glucosa cerebral post-estimulación anóxica de los cuerpos carotídeos en ratas

The commissural nucleus of the solitary tract (NTSc) is the relay center of the afferents fibers from the carotid baro and chemoreceptors, so that modulates blood pressure and blood sugar to stimuli in these receptors. Anoxic stimulation with sodium cyanide (NaCN) in the carotid bodies produces a hyperglycemic response. Somatostatin (SS) inhibits secretion of growth hormone and glucagon producing a hypoglycemic effect. The SS and its receptors in the NTS have an inhibitory effect. It is postulated that somatostatin modulates the hyperglycaemic response after stimulation of carotid chemoreceptors (QRC) with NaCN. In this work, the SS infusion into NTSc 4 min before the anoxic stimulation of the QRC, decreased the hyperglycemic reflex and cerebral glucose retention after 10 min of anoxic stimulus. We conclude that SS modulates the NTSc hyperglycemic response and brain glucose retention post-anoxic stimulation of the carotid bodies in rats

Viability and molecular authentication of Coccidioides spp. isolates from the Instituto de Medicina Tropical de São Paulo culture collection, Brazil / Viabilidade e autenticação molecular de isolados de Coccidioides spp. da coleção de culturas do Instituto de Medicina Tropical de São Paulo, Brasil

Coccidioidomycosis is an emerging fungal disease in Brazil; adequate maintenance and authentication of Coccidioides isolates are essential for research into genetic diversity of the environmental organisms, as well as for understanding the human disease. Seventeen Coccidioides isolates maintained under mineral oil since 1975 in the Instituto de Medicina Tropical de São Paulo (IMTSP) culture collection, Brazil, were evaluated with respect to their viability, morphological characteristics and genetic features in order to authenticate these fungal cultures. Only five isolates were viable after almost 30 years, showing typical morphological characteristics, and sequencing analysis using Coi-F and Coi-R primers revealed 99% identity with Coccidioides genera. These five isolates were then preserved in liquid nitrogen and sterile water, and remained viable after two years of storage under these conditions, maintaining the same features.

Coccidioidomicose é uma doença emergente no Brasil; a manutenção adequada e autenticação de isolados de Coccidioides spp são essenciais para a pesquisa em diversidade genética de micro-organismos, bem como para a compreensão da doença em humanos. Dezessete isolados de Coccidioides preservados em óleo mineral desde 1975 na coleção de culturas do Instituto de Medicina Tropical de São Paulo (IMTSP) foram avaliados com relação à viabilidade, características morfológicas e genéticas, com o objetivo de autenticação das culturas fúngicas. Dos 17 isolados, apenas cinco foram viáveis após quase 30 anos mantidos em óleo mineral, apresentando características morfológicas e moleculares típicas do gênero, o sequenciamento utilizando os oligonucleotídeos Coi-F e Coi-R revelou identidade de 99% com isolados de Coccidioides. Estes cinco isolados foram preservados em nitrogênio líquido e água destilada esterilizada, e permaneceram viáveis após dois anos de armazenamento sob estas condições, mantendo as mesmas características.

Participación del óxido nítrico, proteína Fos y el tallo cerebral en la retención de glucosa encefálica durante la hipoxia / Involvement of the nitric oxide, Fos protein and brain stem in the retention of brain glucose during hypoxia

Se ha descrito que el núcleo del tracto solitario (NTS), estructura del tallo cerebral y vía de relevo de las aferencias del los quimiorreceptores del senocuerpo carotídeo (RSCC), participa en el aumento en la retención de glucosa por el cerebro (RGC) ante una hipoxia. Es probable que en esta respuesta participe el óxido nítrico (NO) y la proteína Fos. En este trabajo se analiza el papel del NO en el NTS sobre la modificación de la RGC y la expresión de la proteína inmunorreactiva Fos (Fos-ir) en ratas in vivo. La inyección de un donador del NO como es el nitroprusiato de sodio (NPS) en el NTS, 4 min antes de la estimulación de los RSCC, disminuyó la RGC, pero incrementó la expresión de Fos-ir en un mayor número de neuronas en el NTS con respecto a las ratas control, que sólo recibieron líquido cefalorraquídeo artificial (LCRa) antes de la estimulación RSCC. En contraste, un inhibidor selectivo del NO como el N?-nitro-L-arginina metil éster (L-NAME) en el NTS 4 min antes de la estimulación RSCC con NaCN, aumentó la RGC, pero disminuyó el número de neuronas Fos-ir comparados con el control o con NPS. La detección inmunohistoquímica de la expresión de Fos-ir en las células del tallo cerebral indica que la estimulación RSCC activa vías dependientes de NO en el NTS, para regular la RGC. El estudio de esta población de células en el NTS, serß importante para definir su caracterización.

It has been said that the nucleus tractus solitarii (NTS), one structure of the brain stem and path of apherences of chemoreceptors of carotid sinus-body, is involved in the increased glucose retention by the brain in case of hypoxia. It is likely that nitric oxide and Fos protein also take part in this response. This paper analyzes the role of nitric oxide in the NTS on the change of glucose retention by the brain and the expression of inmunoreactive protein Fos (ir-Fos) in rats in vivo. The injection of a NO donor such as sodium nitroprusiate in the NTS four minutes before the stimulation of carotid sinus-body chemoreceptors decreased glucose retention by the brain but increased the expression of ir-Fos in a higher number of neurons in NTS with respect to control group rats which only received artificial cerebrospinal fluid before the stimulation. In contrast, the use of a selective NO inhibitor such as NO-nitro-L-arginine methyl ester (L-NAME) in the NTS four minutes before the stimulation of the chemoreceptors with NaCN, increased the glucose retention by the brain but reduced the number of neurons with ir-Fos expression when compared with the control group or the sodium nitroprusiate injection. The immunohistochemical detection of ir-Fos expression in the brain stem cells indicated that stimulation of carotid sinus-body chemoreceptors activated NO-dependent paths in the NTS to regulate glucose retention by the brain. The study of this cell population in the NTS will be important to define its characterization.

Reflexiones sobre las relaciones ciencia, tecnología, sociedad en el curso del proceso de investigación del óxido nítrico / Reflections on the relationships between science, technology, and society throughout the research process of nitric oxide

Se abordan la interrelación dialéctica entre la ciencia, la tecnología y la sociedad, y los estudios sobre el óxido nítrico en la fase preclínica. Los estudios de la ciencia, la tecnología y la sociedad permiten comprender la responsabilidad ética y social del científico, quien la asume desde el comienzo del proceso con la elaboración de la idea primaria que luego da lugar al proyecto, hasta su culminación con la difusión e implementación de los resultados. Se analizan algunas de las condicionantes sociales de la investigación en el contexto de América Latina. Se señala la necesidad del uso del óxido nítrico como neuromodulador en la respuesta hiperglucemiante en la fase de investigación aplicada.

We approach the dialectical interrelation between science, technology, and society and the pre-clinical trials of nitric oxide. The studies on science, technology, and society help understanding the scientist’s ethical and social responsibility assumed since the beginning when the primary idea is elaborated to later become a project, until its termination with the diffusion and implementation of results. Within the Latin American context, we analyzed some of the social determining factors of this research. We support the necessary use of nitric oxide as a neuromodulator on the hyperglycemic response in the applied research phase.

Correlación clínico-patológica en entidades dermatológicas. Clínica Ces Medellín 1995-1997 / Clinic-patological correlation in dermatologicals diseases: Clinica Ces Medellin 1995-1997

A diario el dermatólogo se enfrenta con la tarea de hacer un buen diagnostico clínico, pero ¿cuántos de éstos se correlacionan en una forma adecuada con el estudio anatomopatológico? Para evaluar dicha concordancia se planteó este estudio, además se buscaba comparar el acierto de los dermatólogos con los de los médicos de otras especialidades. El presente es un estudio analítico buscando la correlación entre los diagnósticos clínicos y los de anatomía - patológica de todas las biopsias procesadas en la Clínica CES durante el período comprendido entre enero de 1995 y mayo de 1997. Para tal fin se revisaron todas las órdenes de solicitud de biopsias y los informes de patología respectivos. En total se tabularon 1198 muestras. La concordancia diagnóstica de los clínicos con los diagnósticos realizados mediante patología fue de 72,3% para los dermatólogos, 11% para los cirujanos plásticos y 16,7% para el grupo de otros médicos. En los tumores benignos no hubo diferencia significativa en la coincidencia diagnóstica entre las diferentes especialidades. La coincidencia entre dermatólogos y otros en los tumores benignos es muy semejante (55,9% y 53,2%); en las entidades infecciosas el grupo de otros médicos tuvo mejor correlación que los dermatólogos. Sería interesante realizar un trabajo por diagnósticos específicos y comparar con otros médicos, para evaluar la causa real de la poca correlación de los dermatólogos y el mejor desempeño de otros especialistas en entidades infecciosas y tumorales.

Everyday the dermatologist faces the responsibility of making a good clinical diagnosis, but how many of these are appropriately correlated with the anatomopathological studies? This study was made to evaluate such correlation, and to see how good are the diagnosis made by dermatologists compared to other specialists. This descriptive study is looking for the concordance between clinical diagnoses and the anatomy – pathology biopsies processed at the CES Clinic during the period between January 1995 and May 997. For such aim all the biopsy requests and the respective information of pathology were reviewed. 1198 samples were tabulated. The concordance viewed between clinical diagnoses and those made through pathology was of 72.3% for the dermatologists, 11% for plastic surgeons and 16.7% for the group of other physicians. In the benign tumors there was no significant difference in the diagnostic concordance among the different specialties. The coincidence between dermatologists and others in the benign tumors is very similar with 55.9% and 53.2%, respectively; in the infectious entities the group of other doctors had better correlation than the dermatologists. It would be interesting to conduct a specific diagnostic research and compare with other physicians, to assess the real cause of the low correlation of dermatologists and better perform of other specialistsin infectious and tumor entibies.

Evaluation of phenotypic and genotypic alterations induced by long periods of subculturing of Cryptococcus neoformans strains

Cryptococcus neoformans is an encapsulated fungal organism that can cause disease in apparently immunocompetent, as well as immunocompromised, hosts. Since 1930, successive subculture has been used to preserve C. neoformans isolates in our Fungus Collection. In the 1970s, some of these Fungus Collection samples were selected to be subjected to a different methods of maintenance - that of lyophilized. Our objective was to analyze C. neoformans isolates in order to make a comparative evaluation between these two methods of preservation. The overall aim of this study was to qualify the preservation technique used in our mycology laboratory since the technique used might affect the survival, stability and purity of the primary isolates in culture. The samples were analyzed using classical mycology methods and using the randomly amplified polymorphic DNA technique In the analysis of phenotypes and genotypes, the typical characteristics of C. neoformans were found to differ in relation to the different methods of preservation employed. The aim of this study was to demonstrate the importance of selecting the appropriate method of preservation for fungus collections. This selection can affect the survival and purity of the cultures, and preserve the stability of their physiological, biochemical, and genetic characteristics.

Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis

Chromoblastomycosis (CBM) is a chronic subcutaneous infection caused by several dematiaceous fungi. The most commonly etiological agent found in Brazil is Fonsecaea pedrosoi, which appears as thick walled, brownish colored cells with transverse and longitudinal division in the lesions, called "muriform cells". This disease is found worldwide but countries like Madagascar and Brazil have highest incidence. Diagnosis is made by clinical, direct and histopathologic examination and culture of specimens. Serological tests have been used to identify specific antibodies against Fonsecaea pedrosoi antigens, as well as immunotechniques have been used for CBM serological identification and diagnosis. In the present study double immunodiffusion (DID), counterimmunoelectrophoresis (CIE) and immunoenzymatic test (ELISA) have been used to evaluate humoral immune response in patients with CBM caused by F. pedrosoi. Metabolic antigen was used for immunoprecipitation tests (DID and CIE) while somatic antigen for ELISA. Our results demonstrated 53 percent sensitivity and 96 percent specificity for DID, while CIE presented 68 percent sensitivity and 90.5 percent specificity. ELISA demonstrated 78 percent sensibility and 83 percent specificity. Serological tests can be a useful tool to study different aspects of CBM, such as helping differential diagnosis, when culture of the pathogenic agent is impossible.

Atypical disseminated cutaneous histoplasmosis in an immunocompetent child, caused by an aberrant variant of Histoplasma capsulatum var. capsulatum

O presente trabalho registra caso de histoplasmose em paciente de 5 anos, HIV negativo, natural e procedente da cidade da Sao Paulo, com lesoes cutaneas nao diagnosticadas clinicamente. Exame histopatologico negativo para infeccao fungica. Cultivos em duas ocasioes, positivos para Histoplasma capsulatum var. capsulatum (amostras 361 e 387). Sorologia negativa para anticorpos anti-Histoplasma capsulatum e Paracoccidioides brasiliensis pelas provas de Imunodifusao dupla e ...

Paracoccidioides brasiliensis: a mycologic and immunochemical study of two strains

Os autores estudaram do ponto de vista micologico, imunoquimico e de sua biologia molecular, duas amostras de Paracoccidioides brasiliensis, uma isolada do solo, no municipio de IBIA (MG) por Silva-Vergara et al (1996,1998) denominada IBIA e outra, BAT, cultivada de um caso humano de paracoccidioidomicose em Ribeirao Preto (SP) por Freitas da Silva (1996). Tais amostras apresentam colonias cotonosa (M) e leveduriforme (L ou Y), sendo patogenicas para cobaios inoculados por via testicular, produzindo orquite granulomatosa e/ou supurativa. Do ponto de vista imunoquimico, atraves de provas de Imunodifusao dupla, Imunoeletroforese e Western Blotting, foi demostrada a presenca da gp43

Paracoccidioides cerebriformis Moore, 1935: mycologic and immunochemical study

O presente estudo trata dos resultados obtidos, do ponto de vista micologico e imunoquimico, de duas amostras de Paracoccidioides consideradas como pertencentes a espécie cerebriformis, criada por MOORE em 1935 e mantidas desde aquela época, através de repiques em agar-Sabouraud, na Micoteca do Instituto de Medicina Tropical de Säo Paulo. Após cerca de 60 anos, tais amostras conservavam as mesmas características descritas por MOORE (1935). Näo foram registradas lesöes experimentais em cobaias inoculadas por via intratesticular, näo se demonstrando também, pelas técnicas SDS PAGE e Western blotting...

Reactivity of anti-gp43 antibodies from Paracoccidioides brasiliensis antiserum with extracts from cutaneous lesions of Lobo's disease: preliminary note

Atraves de varias provas imunoquimicas foi demonstrada a presenca da gp43 em extratos de lesoes cutaneas da doenca de Jorge Lobo. A glicoproteina de 43 kDa e um dos antigenos dominantes do Paracoccidioides brasiliensis. permitindo a identificacao desta especie fungica

Paracoccidioides brasiliensis: a mycologic and immunochemical study of a sample isolated from a armadillo (Dasipus novencinctus)

Amostra de Paracoccidioides brasiliensis isolada de visceras (baco e figado) de um tatu (Dasipus novencintus)foi estudada no ponto de vista micologico e imunoquimico. O tatu havia sido capturado em area da usina hidroeletrica de Tucurui (Estado do Para). Este ja havia sido considerado como reservatorio enzootico do Paracoccidioides brasiliensis naquela regiao. Esta amostra, conservada na Micoteca do Instituto de Medicina Tropical de Sao Paulo sob o numero 135, apresenta todas as caracteristicas de Paracoccidioides brasiliensis, com elevado poder antigenico e baixa virulencia para cobaios e ratos Wistar. A demonstracao do exo-antigeno especifico do P. brasiliensis, representado pela glicoproteina de peso molecular 43 KDa, foi evidente atraves das tecnicas de Imunodifusao Dupla, Imunoeletroforese, SDS-PAGE e Imunoblotting.